Coding

Part:BBa_K1430003:Design

Designed by: Cedric Blötz   Group: iGEM14_Goettingen   (2014-09-30)


yeast TRP1 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated the restriction site for Xba1 in the sequence that is gets compatibel to Prefix/Suffix enzymes. We used PCR approch with primers that add a different base in the recognition site


Source

genomic DNA of S. cerevisiae

References